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dc.contributor.authorUluışık, İrem
dc.contributor.authorKarakaya, Hüseyin Çaǧlar
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2020-12-15T10:51:22Z
dc.date.available2020-12-15T10:51:22Z
dc.date.issued2020en_US
dc.identifier.citationUluisik, İ., Karakaya, H.C. & Koc, A. (2020). tRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Efflux. Molecular Biology, 54 (3), 396–401. https://doi.org/10.1134/S0026893320030188en_US
dc.identifier.urihttps://doi.org/10.1134/S0026893320030188
dc.identifier.urihttps://hdl.handle.net/20.500.12508/1504
dc.description.abstractBoric acid is essential for plants and has many vital roles in animals and microorganisms. However, its high doses are toxic to all organisms. We previously screened yeast deletion collections to identify boric acid-resistant and susceptible mutants to identify genes that play a role in boron tolerance. Here, we analyzed boron resistant mutants (elp1 increment ,elp3 increment ,elp6 increment ,ncs2 increment ,ncs6 increment andkti12 increment ) for their abilities to modulate the general amino acid control system (GAAC) and to induce boron efflux pumpATR1. The mutants analyzed in this study lack the genes that play roles in tRNA Wobble base modifications. We found that all of the boron resistant mutants activated Gcn4-dependent reporter gene activity and increased the transcript level of theATR1gene. Additionally, boron resistant cells accumulated less boric acid in their cytoplasm compared to the wild type cells upon boron exposure. Thus, our findings suggested that loss of wobble base modifications in tRNA leads to GAAC activation andATR1induction, which in turn reduced intracellular boron levels and caused boron resistance.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1134/S0026893320030188en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBoronen_US
dc.subjectBoric aciden_US
dc.subjecttRNAen_US
dc.subjectWobble baseen_US
dc.subjectBoron resistanceen_US
dc.subjectElongator complexen_US
dc.subjectYeasten_US
dc.subjectGeneral amino acid controlen_US
dc.subject.classificationBiochemistry & Molecular Biology
dc.subject.classificationBoron | Plant Micronutrients | Poncirus Trifoliata
dc.subject.otherRhamnogalacturonan II
dc.subject.otherOxidative stress
dc.subject.otherDietary boron
dc.subject.otherTransporter
dc.subject.otherTranslation
dc.subject.otherTolerance
dc.subject.otherExporter
dc.subject.otherCleavage
dc.subject.otherRequires
dc.subject.otherCancer
dc.titletRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Effluxen_US
dc.typearticleen_US
dc.relation.journalMolecular Biologyen_US
dc.contributor.departmentMühendislik ve Doğa Bilimleri Fakültesi -- Biyomedikal Mühendisliği Bölümüen_US
dc.identifier.volume54en_US
dc.identifier.issue3en_US
dc.identifier.startpage396en_US
dc.identifier.endpage401en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.isteauthorUluışık, İrem
dc.relation.indexWeb of Science - Scopus - PubMeden_US
dc.relation.indexWeb of Science Core Collection - Science Citation Index Expanded


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