Effects of Nitrogen and Phosphorus Concentrations on the Growth and Lipid Accumulation of Microalgae Scenedesmus obliquus

Abstract

In the study, Scenedesmus obliquus green algae was cultivated under laboratory conditions at 21±2°C, 16:8 (light:dark) photoperiod and continuous aeration in different nitrogen and phosphorus ratio nutrient medium and its growth was determined. Dry weight, cell density (optical density) and chlorophyll a and b were used to determine the growth of the algae. The best growth was determined in the group consisting of 30 ml NaNO3+10 ml PO4. The amount of biomass obtained was determined as 1.549 gL-1 in this group. The lowest values were the group containing 5 ml NaNO3+5 ml PO4. With the decrease in the amount of nitrogen in the medium, an increase in the amount of carotene and a decrease in the amount of chl a and b were detected. The highest lipid values were determined as 36.7% in the group containing 5 ml NaNO3+5 ml PO4 and 36.2% in the group containing 5 ml NaNO3+10 ml PO4.