Isolation and Characterization of Collagen from the Invasive Sea Urchin (Diadema setosum L., 1778) in North-Eastern Mediterranean Sea, Türkiye

Abstract

In the present study, collagen was obtained from tissues of the invasive sea urchin (Diadema setosum) rapidly spread in the Mediterranean Sea. As a result, the yield of collagen isolated from sea urchin was determined to be 23.78 +/- 1.33% (dry weight). As a result of SDS-Page analysis, it was determined that it contains (alpha 1)2 alpha 2, (Molecular Weight (MA) 124, 114 kDa) and 13 chains (MA 245 kDa) similar to calfskin collagen. In the amino acid analysis of collagen, it was determined that the imino acid (proline+hydroxyproline) content was 196.1 residues/1000 residues. The functional bands of amide A (3301 cm-1), B (2924 cm-1), I (1643 cm-1), II (1550 cm-1), and III (1242 cm-1) functional bands were visualized in the FTIR spectrum. These results were like other collagen sources. Invasive sea urchin was used as a source of collagen for the first time in the present study. An alternative source of collagen to mammalian collagen, which is used commercially in many industries such as biomedicine, food and cosmetics, was isolated for the first time from D. setosum. It was proposed that marine collagen can be used as an alternative source of collagen and a functional component in areas including food, cosmetics, and pharmaceutical industries.

Bu çalışmada, Akdeniz'de hızla yayılan istilacı denizkestanesi (Diadema setosum) dokularından kolajen elde edilmiştir. Sonuç olarak, denizkestanesinden elde edilen kolajenin verimi %23.78±1.33 (kuru ağırlık) olarak belirlenmiştir. SDS-Page analizi sonucunda, dana derisi kolajenine benzeyen (α1)2α2, (Molekül Ağırlığı (MA) 124, 114 kDa) ve ß zincirleri (MA 245 kDa) içerdiği tespit edilmiştir. Kolajenin amino asit analizinde imino asit (prolin+hidroksiprolin) içeriğinin 196.1 kalıntı/1000 kalıntı olduğu belirlenmiştir. FTIR spektrumuna göre Amid A (3301 cm-1), Amide B (2924 cm-1), Amide I (1643 cm-1), Amide II (1550 cm-1) ve Amide III (1242 cm-1) fonksiyonel bantları görüntülenmiştir. İstilacı denizkestanesinden kolajen ilk defa bu çalışma ile elde edilmiştir. Biyotıp, gıda ve kozmetik gibi birçok endüstride ticari olarak kullanılan memeli kolajenine alternatif olarak istilacı denizkestanesinden (D. setosum) kolajen ilk kez elde edilmiştir. Elde edilen bu kolajeninin gıda, kozmetik ve ilaç endüstrileri gibi alanlarda alternatif bir kolajen kaynağı ve fonksiyonel bir bileşen olarak kullanılabileceği önerilmiştir.